Individual Variability of Salivary Gland Proteins in Three Phlebotomus Species

Publication type: 

EDENext Number (or EDEN No): 

EDENext003

Authors: 

Iva Rohousova; Vera Volfova, Sona Skarupova, and Petr Volf

Bibliography Partner: 

Journal: 

Status: 

Year: 

2011

Reference: 

Acta Trop. 2012 Apr;122(1):80-6. Epub 2011 Dec 16.

Host: 

Pathogen: 

Data description: 

Salivary protein profiles

Keywords: 

Keywords: Salivary gland, Phlebotomus

Abstract: 

Pooled salivary gland samples are frequently used to ensure the sufficient amount of material for the experiments; however, this could mask an individual variability. Thus, we compared salivary protein profiles in seven colonies of three Phlebotomus species: Phlebotomus sergenti, Phlebotomus perniciosus, and Phlebotomus papatasi. Surprisingly, the individual profiles differed significantly between the colonies as well as between individuals. The highest variability was observed in proteins with molecular masses of 42-46kDa corresponding to the yellow-related proteins. The phenogram constructed from salivary gland profiles revealed the existence of two main groups in P. sergenti, corresponding well with the geographical origin. The F1 progeny obtained from cross-mating studies between P. sergenti colonies of different geographical origin formed a distinct subgroup within the parental groups. In P. papatasi, several groups of protein profiles were observed with no relationship to the geographical origin. The biological role of salivary proteins variability is discussed.